目的:探讨同型半胱氨酸(HCY)与雄激素性脱发(AGA)的关系。
方法:对小鼠进行病例对照研究和两个观察性实验。在第一部分,共纳入528例中国AGA患者和500例年龄匹配的健康对照.比较AGA和对照组的血清HCY水平。在第二部分,八只小鼠分为两组。两组小鼠都去除了毛发。AGA组接受DHT注射,另一个作为对照组。通过ELISA检测毛囊(HFs)中的HCY水平并进行比较。在第三部分,将12只小鼠分为3组,分别饲喂不同浓度的蛋氨酸。4周后,血清HCY水平,通过对毛发生长相关参数的观察和HE染色,和免疫组织化学(IHC)毛发生长相关标志物Ki67,VEGF,比较3组的IGF-1、Krt27、FGF9和TGF-β1。
结果:在第一部分中,AGA中的HCY水平高于两种性别的对照组。然而,不同严重程度组之间HCY水平无差异.AGA患者高同型半胱氨酸血症的发生率高于对照组。Logistic回归分析显示血清HCY水平与AGA发生率呈正相关。在第二部分,AGA组HFs的HCY明显高于对照组。第三部分显示血清HCY水平的升高抑制了小鼠毛发的生长,与表达较少的刺激标志物Ki67,VEGF,IGF-1,Krt27和FGF9,而抑制性标记TGF-β1的表达没有差异。
结论:HCY和AGA之间存在潜在的关系。HCY对毛发生长具有抑制作用。具体机制有待进一步研究探讨。
OBJECTIVE: To investigate the relationship between homocysteine (HCY) and androgenetic
alopecia (AGA).
METHODS: A case control study and two observational experiments on mice were conducted. In the first part, a total of 528 Chinese AGA patients and 500 age-matched healthy controls were included. Serum HCY levels of AGA and controls were compared. In the second part, eight mice were divided into two groups. Both groups of mice had their hair removed. AGA group received a DHT injection, and the other as control group. HCY levels in hair follicles (HFs) were detected by ELISA and compared. In the third part, twelve mice were divided into three groups and fed with different concentrations of methionine. After 4 weeks, serum HCY levels, parameters related to hair growth through observation and HE staining, and expression of immunohistochemistry (IHC) hair-growth-related markers Ki67, VEGF, IGF-1, Krt27, FGF9, and TGF-β1 were compared among the three groups.
RESULTS: In the first part, HCY levels were higher in AGA than the controls of both genders. However, there was no difference in HCY levels between groups with varying severity. Rates of hyperhomocysteinemia was higher in AGA patients than the controls. Logistic regression analysis showed serum HCY levels was positively correlated with the incidence of AGA. In the second part, HCY of the HFs in the AGA group was significantly higher than that in the control group. The third part showed that the increase in serum HCY levels inhibited the growth of mice hair, with the less expressed stimulative markers Ki67, VEGF, IGF-1, Krt27, and FGF9, while there was no difference in the expression of inhibitory markers TGF-β1.
CONCLUSIONS: There is a potential relationship between HCY and AGA. HCY had an inhibitory effect on hair growth. Further studies are necessary to explore the specific mechanism.